[Download] "Automated Turbidimetric Benzalkonium Chloride Method for Measurement of Protein in Urine and Cerebrospinal Fluid (Technical Briefs)" by Clinical Chemistry * Book PDF Kindle ePub Free

📘 Read Now     📥 Download

eBook details

• Title: Automated Turbidimetric Benzalkonium Chloride Method for Measurement of Protein in Urine and Cerebrospinal Fluid (Technical Briefs)
• Author : Clinical Chemistry
• Release Date : January 01, 2004
• Genre: Chemistry,Books,Science & Nature,
• Pages : * pages
• Size : 190 KB

Description

Various methods have been used to measure protein in urine and cerebrospinal fluid (CSF). Urinary protein can be determined by turbidimetric assays using trichloroacetic acid (1), sulfosalicylic acid (2), or benzethonium chloride (BTC) (3, 4); the biuret assay (5, 6); or protein dyebinding assays using Coomassie Brilliant Blue (CBB) (7-13), Pyrogallol Red (PYR)-molybdate (14,15), or Ponceau S (16). The BTC, CBB, and PYR methods can be automated. Shephard and Whiting (17) used benzalkonium chloride (BC) as a precipitating agent for the determination of protein in urine and CSF by nephelometry. In this study we developed an automated turbidimetric method using BC and compared it with conventional automated methods. Albumin fraction V (bovine), disodium molybdate x 2 [H.sub.2]O, NaOH, sodium azide, sodium benzoate, potassium oxalate, and succinic acid were obtained from Merck; BTC, Coomassie Brilliant Blue G 250, EDTA, and PYR were from Sigma; BC was from Serva; and phosphoric acid was from Riedel de Haen. A commercially available PYR reagent (M-TP) and protein calibrators were obtained from Beckman Coulter. A globulin solution containing mostly IgG and IgM and no albumin was obtained from the Refik Saydam Center of Hygiene, Turkey.

Free Download "Automated Turbidimetric Benzalkonium Chloride Method for Measurement of Protein in Urine and Cerebrospinal Fluid (Technical Briefs)" PDF ePub Kindle